Anticancer and antioxidant activities of ethanolic extract and semi-purified fractions from guava and mango seeds

Abstract Mango ( Mangifera indica ) and guava Psidium guajava seeds have several pharmaceutical applications biological activities because as they been recognized with different bioactive molecules (phenolic compounds) such flavonoids, phenolic acids, catechins, so antioxidant anticancer activities. The aim of the present study was to assess in vitro successive extracts semi-purified fractions from mango seeds. In this work, were collected extracted using two solvents (ethanol 70% ethyl acetate) followed by phytochemical screening determination activity five assays (DPPH, ABTS, KMnO 4 , Methylene blue H 2 O additionally antiradical hybrid reaction for ethanolic extract promising extract. total phenolic, flavonoid, catechin compounds determined all extracts, finally, evaluated MTT assay against HepG2 cell line identified HPLC. TLC showed primary investigation ethanol both kind only acetate extracts. However, HPLC these three high amount gallic acid, naringenin, ellagic ferulic acid tests that is highest DPPH 84.0%, but recorded 82.0% methylene ABTS when compared ascorbic acid. strong cytotoxic effect IC 50 75.5 μg/mL tested concentrations. From obtained results, it could be concluded its are most antioxidants activity.